ABSTRACT
<p><b>OBJECTIVE</b>To clarify the role of midkine (MK) in vulvar carcinogenesis though examination of its expression in vulvar lesions including vulvar condyloma acuminata (VCA), vulvar intraepithelial neoplasia (VIN) and vulvar squamous cell carcinomas (VSCC), and to analyze the relationship between MK expression and human papilloma virus (HPV) infection.</p><p><b>METHODS</b>Thirty VSCC, 15 VIN and 10 VCA patients were studied by streptavidin-biotin-immunoperoxidase method. MK expression was compared with clinicopathologic features of vulvar tumors.</p><p><b>RESULTS</b>MK was expressed in 26 of 30 VSCC (87%), 3 of 5 VIN III and all VCA samples, whereas no MK expression was detected in the VIN I-II samples or in normal epithelium. The difference of MK expression between VIN III and VSCC was statistically significant (P < 0.05). MK was more intensely expressed in differentiated-type (well differentiated and moderately differentiated) VSCC than in undifferentiated-type (poorly differentiated) VSCC. There was no statistically significant correlation between MK expression and clinical stage, lymph node metastasis and HPV infection in VSCC. MK expression were observed in all HPV-positive specimens including 2 VSCC, 1 VIN III and all VCA.</p><p><b>CONCLUSIONS</b>MK gene expression may be a late event in vulvar squamous cell malignant transformation, and may be associated with vulvar tumor cell differentiation. HPV-positive vulvar tumors expressed MK protein.</p>
Subject(s)
Female , Humans , Carcinoma, Squamous Cell , Chemistry , Virology , Carrier Proteins , Condylomata Acuminata , Metabolism , Virology , Cytokines , Papillomaviridae , Chemistry , Papillomavirus Infections , Metabolism , Precancerous Conditions , Chemistry , Virology , Tumor Virus Infections , Metabolism , Vulvar Diseases , Metabolism , Virology , Vulvar Neoplasms , Chemistry , VirologyABSTRACT
Objective To study the effects of estradiol-17β (17β-E2) on the synthesis of GnRH and GH in submandibular gland of rat. Method Immunohistochemical ABC method was used to observe the localization of GnRH and GH in submandibular gland of rat.Results The synthsis of GnRH was promoted and the synthesis of GH was inhibited by 17β-E2 in rat's submandibular gland. Conclusion The 17β-E2 may play an important regulative role in the synthesis of GnRH and GH.
ABSTRACT
Objective: Our aim was to study the role of telomerase activation in the course of cervical carcinogenesis and progression.Methods:Telomeric repeat amplification protocol(TRAP) assay was used to measure telomerase activity in tissue samples with various cervical conditions:40 with cervical cancer, 50 with cervical intraepithelial neoplasia(CIN), 20 with normal cervice. Results:The positive rate of telomerase activity was 95.0%,44.0%, and 10.0% in cervical cancer, CIN, and normal cervices, respectively, which was significantly higher in cervical cancer than that in CIN and normal cervices, so was that in CIN than that in normal cervices (P<0.01) . The positive rate was 22.2%, 37.5%, and 75.0% in CINⅠ,Ⅱ, and Ⅲ, respectively, which was significantly higher in CINⅢ than that in CIN Ⅱand CINⅠ (P<0.01).Conclusion:Telomerase activation may relate to cervical carcinogenesis, which correlates well with the grade of cervical lesions.